HETEROKARYOSIS IN STREPTOMYCES

Publisher Website
Google Scholar
Abstract
Heterokaryosis was established in combinations of S. griseus and S. griseus X S. cyaneus by analyzing prototrophic colonies Formed from intimately associated combinations of complementary auxotrophs on minimal agar. Spores from prototrophic colonies are unable to propagate that character whereas mycelial fragments often can. Genetically, the spores are homokaryotic, corresponding to one or the other parental type. Heterokaryosis of the vegetative mycelium was demonstrated by presence of both intact parental genomes in a single hyphal fragment isolated by micromanipulation. In-frequent "anomalous heterokaryons" that can not synergize effectively even though both parental genomes are present were found. No stable recombinants were detected in these experi-ments nor in survey experiments designed especially for that purpose.