Structure of the fructose-containing K52 capsular polysaccharide of uropathogenic Escherichia coli O4:K52:H−

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Abstract
The chemical structure of the K52 antigenic capsular polysaccharide (K52 antigen) of E. coli O4:K52:H- was elucidated by composition, NMR spectroscopy, methylation, periodate oxidation before and after graded acid hydrolysis and by oligosaccharide analysis. The polysaccharide consists of a backbone of .alpha.-galactose units interlinked between C1 and C3 by phosphodiester bridges. This poly(.alpha.-galactosylphosphate) is substituted at C2 of each galactose unit by .beta.-fructofuranose residues. About 80% of the galactose units are O-acetylated at C4 and .apprx. 10% of the fructose units are both O-acetylated and O-propionylated at C1. The K52 polysaccharide has an average MW of 34 kilodaltons, thus consisting of .apprx. 65 fructosyl-galactosyl-phosphate repeating units.

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