CHEMICAL-REACTIONS IN NUCLEIC-ACID DUPLEXES .4. CYANOGEN-BROMIDE AS AN EFFICIENT REAGENT IN CONDENSATION OF OLIGODEOXYRIBONUCLEOTIDES
- 1 September 1987
- journal article
- research article
- Vol. 13 (9) , 1286-1288
Abstract
Cyanogen bromide was found to induce a smooth and highly efficient condensation of oligonucleotide blocks within DNA duplexes. This template-directed reaction proceeds in the aqueous solution at 0.degree. C for 1-2 min. Cyanogen bromide catalyzes the formation not only of phosphodiester, but also of unnatural phosphoramide and pyrophosphate interoligomer bonds.This publication has 5 references indexed in Scilit:
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