Suspension cultures of higher plant cells in synthetic media

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Abstract
A relatively simple technique for the cultivation in a completely defined synthetic medium of cell suspensions derived from high plant tissues is described. Methods for studying tissue proliferation and tissue separation are outlined and data from experiments with callus tissues from carrot (Daucus carota) and bindweed (Convolvulus arvensis) are presented. Tissue growth in terms of fresh weight production is best in complex media but is of the same order of magnitude in the synthetic medium described. Tissue separation into free floating cells is dependent upon the auxin concentration in the medium; in the absence of added auxin, separation is markedly reduced. Evidence is presented for the requirement of certain vitamins by the cell suspension cultures of both Convolvulus and carrot for maximum growth; these are requirements which are not manifest by these tissues when grown on solidified medium. The potential usefulness of the cell suspension technique using synthetic media is discussed.

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