INTERLABORATORY SURVEY OF ENZYME ANALYSES .1. PRELIMINARY STUDIES

  • 1 January 1976
    • journal article
    • research article
    • Vol. 66  (1) , 206-222
Abstract
Enzyme survey specimens were prepared by spiking portions of a normal human serum pool with creatinine, urea and 5 enzymes (lactate dehydrogenase, creatine phosphokinase, alkaline phosphatase, and aspartate and alanine aminotransferases), and preparing admixtures of the spiked pools with the original serum. This admixture technique established linear interspecimen relationships that could be confirmed by analyses for creatinine and urea N. Both ethylene glycol-stabilized liquid serum specimens and lyophilized specimens were prepared as sets of 6-8 samples having 6 concentrations of each enzyme. The sets were distributed on 5 occasions to about 10 laboratories that were widely separated geographically, and the specimens were analyzed by a variety of methods, by various instrumental systems, and in different reaction conditions, and results were reported in diverse units. The analytic data obtained through the use of these specimens that were specifically designed for survey purposes can be analyzed statistically to provide meaningful assessments of laboratory performance in enzyme analyses.

This publication has 2 references indexed in Scilit: