Dilatation of Golgi vesicles by monensin leads to enhanced accumulation of sugar nucleotides
- 1 February 1986
- journal article
- research article
- Published by Portland Press Ltd. in Bioscience Reports
- Vol. 6 (2) , 227-234
- https://doi.org/10.1007/bf01115011
Abstract
Incubation of mouse thymocytes with 10μM monensin for 1 hour induces morphological alterations characterized by the extensive dilatation and vacuolization of the Golgi complex. This effect is used to study the transport and utilization of labelled sugar nucleotides into intracellular vesicles by using thymocytes whose plasma membrane has been permeabilized by ammonium chloride treatment. It is demonstrated that monensin stimulates the incorporation of labelled sialyl, fucosyl, galactosyl, and N-acetylglucosaminyl residues. This enhanced incorporation is not due to a direct effect of monensin on glycosyltransferase activities themselves but is a consequence of a higher entry and accumulation of labelled sugar nucleotides in the dilated vesicles.Keywords
This publication has 18 references indexed in Scilit:
- Intralumenal pool and transport of CMP-N-acetylneuraminic acid, GDP-fucose and UDP-galactose. Study with plasma-membrane-permeabilized mouse thymocytesBiochemical Journal, 1984
- Monensin prevents terminal glycosylation of the N- and O-linked oligosaccharides of the HLA-DR-associated invariant chain and inhibits its dissociation from the alpha-beta chain complex.Proceedings of the National Academy of Sciences, 1984
- Glycosylation of proteins from sugar nucleotides by whole cells. Effect of ammonium chloride treatment on mouse thymocytesBiochemical Journal, 1983
- Effects of monensin on the processing of pro-opiomelanocortin in the intermediate lobe of the rat pituitaryBiochemical and Biophysical Research Communications, 1982
- Isolation and properties of .alpha.-D-mannose:.beta.-1,2-N-acetylglucosaminyltransferase from trachea mucosaBiochemistry, 1981
- Intracellular transport of secretory and membrane proteins in hepatoma cells infected by vesicular stomatitis virusCell, 1980
- The kinetics of iron uptake in vitro by human duodenal mucosa: studies in normal subjects.The Journal of Physiology, 1979
- Monovalent ionophores inhibit secretion of procollagen and fibronectin from cultured human fibroblastsProceedings of the National Academy of Sciences, 1979
- Comparative studies of intracellular transport of secretory proteins.The Journal of cell biology, 1978
- Plasma cell immunoglobulin secretion. Arrest is accompanied by alterations the golgi complexThe Journal of Experimental Medicine, 1977