Ribonucleic Acid Polymerase Activity Associated with Purified Calf Rotavirus
- 1 September 1977
- journal article
- research article
- Published by Microbiology Society in Journal of General Virology
- Vol. 36 (3) , 395-402
- https://doi.org/10.1099/0022-1317-36-3-395
Abstract
The presence of an RNA-dependent RNA polymerase is demonstrated in purified rotavirus particles. Optimum polymerase activity was found between 45 to 50 °C, at pH 8, and in the presence of 10 mm-magnesium ions. The polymerase product was highly sensitive to pancreatic RNase (97%) in low or high salt concentration. The enzyme was activated by EDTA treatment of intact particles or heat shock. The similarities between reovirus, blue-tongue virus and rotavirus polymerases are discussed.Keywords
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