Separation of polyunsaturated fatty acids by argentation thin layer chromatography

Abstract
A single step, silver nitrate thin layer chromatographic procedure which separates a methyl ester mixture containing 0–6 double bonds is described. Purity of each ester recovered from the silver nitrate plate was 98–99%. Recovery of the esters ranged from 100% for saturates to 77% for pentaenes.

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