Randomly Distributed DNA Single Strand Breaks are not Lethal for Mammalian Cells
- 1 January 1988
- journal article
- research article
- Published by Taylor & Francis in Xenobiotica
- Vol. 18 (12) , 1481-1487
- https://doi.org/10.3109/00498258809042270
Abstract
1. The primary objective of this study was to assess whether randomly distributed DNA single strand breaks result in cytotoxicity. 2. The experimental approach was exposure of cultured Chinese hamster ovary cells to hydroxyl radicals generated either by X-rays (via water radiolysis) or from H2O2 (via the Fenton reaction). 3. An excellent inverse correlation was observed with X-rays: the higher the level of DNA breakage, the lower the cloning efficiency of the cells. 4. In the case of H2O2 the same correlation was found, however, single strand breaks produced by this agent appeared less toxic than those generated by X-irradiation. 5. Since considerable evidence indicates DNA damage as the major cause of X-ray- and H2O2-induced lethality, it is concluded that randomly distributed single strand breaks have hardly any effect in cell killing.This publication has 6 references indexed in Scilit:
- Effect of 3-aminobenzamide on DNA strand-break rejoining and cytotoxicity in CHO cells treated with hydrogen peroxideBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1986
- Mammalian Cells Are Not Killed by DNA Single-Strand Breaks Caused by Hydroxyl Radicals from Hydrogen PeroxideRadiation Research, 1985
- In vivo formation of single-strand breaks in DNA by hydrogen peroxide is mediated by the Haber-Weiss reactionBiochimica et Biophysica Acta (BBA) - Gene Structure and Expression, 1984
- Comparison of the effects of hydrogen peroxide and X-ray irradiation on toxicity, mutation, and DNA damage/repair in mammalian cells (V-79)Biochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1981