Neuronal protein gene product 9.5 (IEF SSP 6104) is expressed in cultured human MRC‐5 fibroblasts of normal origin and is strongly down‐regulated in their SV40 transformed counterparts

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Abstract
Neuronal protein gene product 9.5 (PGP 9.5) most likely identical to ubiquitin carboxyl‐terminal hydrolase isozyme LI (UCH‐LI) has been reported to be expressed almost exclusively in neuronal and neuroendocrine tissues. By two‐dimensional (2D) immunoblotting, comigration and microsequencing of proteins recovered from 2D gels we have identified PGP 9.5/UCH‐LI as polypeptide IEF SSP 6104 (M r = 27000, pl = 5.49) in the comprehensive 2D gel cellular protein database of human embryonal lung MRC‐5 fibroblasts [(1989) Electrophoresis 10, 76–115; (1990) Electrophoresis 11, 1072–1113]. This protein is expressed at high levels in quiescent and proliferating cultured normal fibroblasts and is strongly down‐regulated (about 10 times) in their transformed counterparts.