Characterization of actin isoforms in ejaculated boar spermatozoa

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Abstract
Actin was localized in boar ejaculated spermatozoa by using specific antisera against cytoplasmic isoforms of actin [Otey et al., J Cell Biol, 102:1726–1737, 1986; Skalli et al., J Cell Biol, 103:2787–2796, 1986; Miller et al., Biochemistry, 26:6064–6070, 1987]. Indirect immunofluorescence and immunoelectron microscopy showed that γ and β actins were codistributed in the acrosomal and postacrosomal regions. Sperm actin was also identified on two-dimensional gel as two spots in the isoelectric point and molecular weight corresponding to β and γ actins. Coelectrophoresis of sperm actin and chicken gizzard actin and immunoblots stained with the specific antibodies confirmed the presence of these two isoforms of actin.