Antitumor Effects of Antibody—Diphtheria Toxin Conjugates. III. Cyclophosphamide-Induced Immune Unresponsiveness to Conjugates2
- 1 September 1975
- journal article
- research article
- Published by Oxford University Press (OUP) in JNCI Journal of the National Cancer Institute
- Vol. 55 (3) , 709-712
- https://doi.org/10.1093/jnci/55.3.709
Abstract
Of 9 hamsters immunized with diphtheria toxin-hamster γ-globulin conjugates and rechallenged with this antigen 15 days later, 8 exhibited neutralizing antibodies against the conjugates 22 days after the initial immunization. Hamsters identically immunized, but also given cyclophos-phamide (CY) 20 hours after the first immunization, had a much lower incidence of positive responses (2/10) and lower antibody levels in the positive responders. After further immunization with the conjugates, all CY-treated hamsters eventually developed neutralizing antibodies, but exhibited a reduction in antibody levels after a second exposure to CY. Hamsters immunized with diphtheria toxoid developed antibodies that were effective in neutralizing diphtheria toxin, but less effective against the diphtheria toxin conjugates.Keywords
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