Rapid isoelectric focusing of plasmid-mediated beta-lactamases with Pharmacia PhastSystem
Open Access
- 1 November 1988
- journal article
- research article
- Published by American Society for Microbiology in Antimicrobial Agents and Chemotherapy
- Vol. 32 (11) , 1730-1732
- https://doi.org/10.1128/aac.32.11.1730
Abstract
A modified isoelectric focusing method for rapid semiquantitative identification of plasmid-mediated beta-lactamases by use of the Pharmacia PhastSystem (Uppsala, Sweden) is described. Sonication of bacterial colonies collected directly from growth plates decreased the time required for the procedure. With sonic extracts of known beta-lactamase-producing strains used as controls, the assay could be completed in less than 2 h.This publication has 4 references indexed in Scilit:
- Detection of plasmid-mediated beta-lactamases with DNA probesAntimicrobial Agents and Chemotherapy, 1988
- Ampicillin resistance in Haemophilus influenzae: identification of resistance mechanismsJournal of Antimicrobial Chemotherapy, 1987
- The Use of Analytical Isoelectric Focusing for Detection and Identification of -LactamasesJournal of General Microbiology, 1975
- Novel Method for Detection of β-Lactamases by Using a Chromogenic Cephalosporin SubstrateAntimicrobial Agents and Chemotherapy, 1972