Involvement of colchicine binding site of tubulin in the polymerisation process
- 7 February 1983
- journal article
- Published by Wiley in FEBS Letters
- Vol. 152 (1) , 101-104
- https://doi.org/10.1016/0014-5793(83)80491-8
Abstract
Role of lysine residues in the colchicine binding site and in the assembly—disassembly process was examined. It was observed that at 4°C (pH 7.5–8, 8±1) lysine residues and the N‐terminal methionine residue of tubulin were all buried within the molecule. Evidence indicates that ϵ‐amino groups of lysine residues of tubulin are shared by both the colchicine binding site and the polymerisation process.Keywords
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