RECOVERY OF VIRUSES FROM 3 TRANSPORT MEDIA INCORPORATED INTO CULTURETTES

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  • 1 January 1981
    • journal article
    • research article
    • Vol. 105  (8) , 436-437
Abstract
A double-blind prospective study was carried out to compare the rates of recovery of viruses from the upper respiratory tracts of children, using 3 different transport media. Stuart''s, Hanks'' and Leibovitz-Emory (LEM) media were packaged in the ampules of Culturettes and labeled in code by the manufacturer. Three swabs (1 each from Culturettes containing different media) were inserted simultaneously into the oropharynx or pressed onto unroofed dermal lesions, transported to the laboratory and inoculated into MRC-5 (human embryonic lung fibroblastic cell) and primary monkey kidney-cell cultures. Eighty isolates were obtained from 200 specimens (40%) collected during all 4 seasons of the year. Parainfluenza virus, enterovirus, adenovirus and herpes simplex virus accounted for 79% of the isolates. Of 80 isolates, 72 (90%) were recovered in Hanks'', 64 (80%) in Stuart''s and 63 (79%) in LEM. The differences were not significant.

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