Environmental Stimuli in the Progression of BHK/21 Cells Through the Cell Cycle
- 1 June 1972
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 69 (6) , 1455-1459
- https://doi.org/10.1073/pnas.69.6.1455
Abstract
An L-cell line adapted to grow in protein-free medium is a source of material that stimulates the proliferation of serum-dependent BHK cells under otherwise protein-free conditions. The material produced by the L-cells is resolvable into separate DNA-synthetic and mitotic stimulatory activities. The stimulation of mitosis is dependent upon the presence of one or more heat-labile macromolecules, which can be concentrated by ultrafiltration, and at least one dialyzable component is essential for the initiation of DNA synthesis.Keywords
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