Isolation of a serum component that stimulates the spreading of cells in culture
- 1 February 1980
- journal article
- research article
- Published by Portland Press Ltd. in Biochemical Journal
- Vol. 185 (2) , 349-354
- https://doi.org/10.1042/bj1850349
Abstract
A fetal-calf-serum component was isolated that stimulates [baby hamster kidney] cells in culture to adopt typical fibroblast morphology. This spreading factor is a glycoprotein with MW of about 62,000 and contains 12% carbohydrate; it has an isoelectric point of 4.8. The method described allows purification to homogeneity as judged by sodium dodecyl sulfate/polyacrylamide-gel electrophoresis and analytical isoelectric focusing. Under cell-culture conditions the factor is active at 18 .mu.g/ml.This publication has 20 references indexed in Scilit:
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