High resolution single strand conformation polymorphism analysis using formamide and ethidium bromide staining.
Open Access
- 1 October 1997
- journal article
- research article
- Published by BMJ in Molecular Pathology
- Vol. 50 (5) , 276-278
- https://doi.org/10.1136/mp.50.5.276
Abstract
Single strand conformation polymorphism (SSCP) analysis using ethidium bromide can be improved by adding formamide as the denaturant. This gives higher resolution than previous SSCP methods; it had 100% sensitivity in the discrimination of 14 PCR samples from two different genes, even for a long fragment close to the upper limit of 250 base pairs. This modified procedure is a rapid, simple, safe, and yet highly sensitive method for detecting structural differences in DNA fragments.Keywords
This publication has 6 references indexed in Scilit:
- ‘Cold SSCP’: a simple, rapid and non-radioactive method for optimized single-strand conformation polymorphism analysesNucleic Acids Research, 1993
- Nonisotopic SSCP detection in PCR products by ethidium bromide stainingTrends in Genetics, 1992
- Nonisotopic SSCP detection in PCR products by ethidium bromide stainingTrends in Genetics, 1992
- Diagnostic single strand conformational polymorphism, (SSCP): a simplified non-radioisotopic method as applied to a Tay-Sachs B1 variantNucleic Acids Research, 1991
- Non-isotopic detection of single strand conformation polymorphism (PCR-SSCP): a rapid and sensitive technique in diagnosis of phenylketonuriaNucleic Acids Research, 1991
- Detection of polymorphisms of human DNA by gel electrophoresis as single-strand conformation polymorphisms.Proceedings of the National Academy of Sciences, 1989