Labeling of Trichogramma pretiosum1 by Rearing on Eggs from 32P-fed Adults of Heliothis zea2,3,4

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Abstract
Heliothis zea (Boddie) female moths were labeled with 32P as orthophosphate using a feeding process and their eggs used as hosts in the rearing of Trichogramma pretiosum Riley. The percentage radioactive eggs oviposited by H. zea moths corresponded almost identically with the percentage radioactive parasites emerging from the eggs. The average counts per minute for the eggs and parasites were closely related with the high 1st day counts, declining progressively on following days of the study. Thus, Trichogramma can be reared on 32P-labeled H. sea eggs without affecting the parasite's ability to further parasitize H. zea eggs under laboratory conditions. There is no difference in the number of parasites emerging from control eggs and 32P-labeled eggs when used in the rearing of Trichogramma pretiosum. Greenhouse data suggest that this process might be used to monitor parasite activities in the field.

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