Evaluation of Two Rapid Immunochromatography Tests for the Detection of Clostridium difficile Toxins
- 8 November 2007
- journal article
- research article
- Published by Springer Nature in Digestive Diseases and Sciences
- Vol. 53 (7) , 1876-1879
- https://doi.org/10.1007/s10620-007-0072-2
Abstract
The aim of the study was to evaluate the performance of two rapid immunochromatography tests, the Tox A/B Quick Chek(R) (TechLab) and the ImmunoCard Toxins A&B test (Meridian), in addition to the older Tox A/B ELISA, against PCR detection of the tcdB gene. Two hundred patient-unique stool specimens were tested. In comparison to the polymerase chain reaction results, the sensitivity and specificity of the two rapid tests were 94.7% and 97.2%, respectively; corresponding values for the ELISA were 93.6% and 94.3%. Findings on the rapid tests showed a 96% correlation with polymerase chain reaction assay and a 94% correlation with ELISA. Considering the importance of early and appropriate diagnosis of Clostridium difficile-associated diarrhea, rapid immunochromatography tests may be of great benefit to practitioners.Keywords
This publication has 15 references indexed in Scilit:
- The Challenges Posed by Reemerging Clostridium difficile InfectionClinical Infectious Diseases, 2007
- Toxin A-negative, toxin B-positive Clostridium difficileInternational Journal of Infectious Diseases, 2007
- Rapid and Simple Method for Detecting the Toxin B Gene of Clostridium difficile in Stool Specimens by Loop-Mediated Isothermal AmplificationJournal of Clinical Microbiology, 2005
- Prospective Multicenter Evaluation of a New Immunoassay and Real-Time PCR for Rapid Diagnosis of Clostridium difficile -Associated Diarrhea in Hospitalized PatientsJournal of Clinical Microbiology, 2005
- Laboratory diagnosis of Clostridium difficile-associated diarrhoea: a plea for cultureJournal of Medical Microbiology, 2005
- Rapid Detection of Clostridium difficile in Feces by Real-Time PCRJournal of Clinical Microbiology, 2003
- High prevalence of toxin A-negative toxin B-positive Clostridium difficile in hospitalized patients with gastrointestinal diseaseDiagnostic Microbiology and Infectious Disease, 2002
- Development and Evaluation of a PCR Method for Detection of the Clostridium difficile Toxin B Gene in Stool SpecimensJournal of Clinical Microbiology, 2002
- Antibiotic-Associated DiarrheaNew England Journal of Medicine, 2002
- Increasing Hospitalization and Death Possibly Due toClostridium difficileDiarrheal DiseaseEmerging Infectious Diseases, 1998