A new form of chromatogram employing two liquid phases

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Abstract
A new form of chromatogram is descr., depending not on adsorption on a solid phase, but on partition of solutes between 2 liquid phases. Visual detection of colorless acids in this chromato-gram can be achieved by adding an indicator to one of the phases. A general theory of chromatography applicable to substance with linear distribution isotherms is developed. This theory is based on the "theoretical plate" concept, thus directly relating chromatography to fractional distilla-tion and extraction. The theory is checked experimentally in the new chromatogram. The new chromatogram is applied to the micro-detn. of the higher monoamino-acids in protein hydrolysates.

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