Functional Expression of the Extraplastidial Arabidopsis thaliana Oleate Desaturase Gene (FAD2) in Saccharomyces cerevisiae
Open Access
- 1 May 1996
- journal article
- Published by Oxford University Press (OUP) in Plant Physiology
- Vol. 111 (1) , 223-226
- https://doi.org/10.1104/pp.111.1.223
Abstract
The functional expression in yeast of the Arabidopsis thaliana FAD2 gene, encoding the extraplastidial oleate desaturase (1-acyl-2-oleoyl-sn-glycero-3-phosphocholine [delta]12-desaturase) is reported. Dienoic fatty acids constituted up to 11% (w/w) of the total fatty acids in transformed Saccharomyces cerevisiae cells and were confirmed to be linoleic acid and [delta]9,[delta]12-hexadecadienoic acid by gas chromatography-mass spectrometry.Keywords
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