In vitro propagation and low temperature storage ofSaussurea lappa C.B. Clarke ? An endangered, medicinal plant
- 1 May 1989
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 8 (1) , 44-47
- https://doi.org/10.1007/bf00735776
Abstract
A procedure forin vitro multiplication ofSaussurea lappa (Asteraceae) is described. On Murashige and Skoog's medium (MS) containing benzylaminopurine and gibberellin 3.5-fold shoot multiplication occurred every three weeks. Shoots rooted on MS containing 0.5 μM naphthaleneacetic acid with 90% efficiency. The shoot cultures stored at 5°C in the dark for 12 months without an intervening subculture survived with 100% viability. The shoots cold stored for 6 months or more showed higher rates of multiplication under culture room conditions than the untreated shoots.Keywords
This publication has 9 references indexed in Scilit:
- An in vitro method for the conservation and storage of garlic (Allium sativum) germplasmPlant Cell, Tissue and Organ Culture (PCTOC), 1987
- Tissue Culture Techniques for Virus Elimination and Germplasm PreservationPublished by Wiley ,1984
- Some Factors Controlling in vitro Morphogenesis of Arabidopsis thalianaZeitschrift für Pflanzenphysiologie, 1978
- Propagation of Chrysanthemum in Vitro. I. Multiple Plantlets from Shoot Tips and the Establishment of Tissue Cultures1,2Journal of the American Society for Horticultural Science, 1974
- CYTOKININ‐GIBBERELLIN REGULATION OF SHOOT DEVELOPMENT AND LEAF FORM IN TOBACCO PLANTLETSAmerican Journal of Botany, 1973
- Some histochemical changes underlying shoot initiation in tobacco callus culturesCanadian Journal of Botany, 1970
- Non-Reversibility of Gibberellin-Induced Inhibition of Regeneration in Begonia LeavesPhysiologia Plantarum, 1969
- The induction of flowering in vitro in stem segments of Plumbago indica L.Planta, 1967
- A Revised Medium for Rapid Growth and Bio Assays with Tobacco Tissue CulturesPhysiologia Plantarum, 1962