Isolation and complete sequence of CBR, a gene encoding a putative cytochrome b reductase in Saccharomyces cerevisiae
- 1 January 1994
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 219 (1-2) , 441-448
- https://doi.org/10.1111/j.1432-1033.1994.tb19957.x
Abstract
We have isolated and characterised a novel yeast gene, CBR (cytochrome b reductase), encoding a 35-kDa yeast novobiocin-binding protein. The predicted protein sequence of CBR displays considerable similarity to both plant nitrate reductases and mammalian cytochrome b5 reductases indicating that it is a putative member of the flavoprotein pyridine-nucleotide-cytochrome-reductase family. Disruption of CBR is not lethal under various growth conditions, suggesting the presence of some functional overlap with other reductases, possibly with the cytochrome P-450 reductase.Keywords
This publication has 68 references indexed in Scilit:
- The yeast type II myosin heavy chain: Analysis of its predicted polypeptide sequenceJournal of Muscle Research and Cell Motility, 1991
- What The Papers Say: Topoisomerase II can relax; novobiocin is a mitochondrial poison after allBioEssays, 1990
- Disruption of the Saccharomyces Cerevlsiae gene for NADPH-cytochrome P450 reductase causes increased sensitivity to ketoconazoleBiochemical and Biophysical Research Communications, 1989
- Selective increase of c‐myc mRNA levels by methylglyoxal‐bis (guanylhydrazone) and novobiocin in serum‐stimulated fibroblastsJournal of Cellular Physiology, 1986
- Tandem Regions of Yeast DNA Topoisomerase II Share Homology with Different Subunits of Bacterial GyraseScience, 1986
- Identification of myosin heavy chain in Saccharomyces cerevisiaeNature, 1985
- Novobiocin blocks the Drosophila heat shock responseJournal of Molecular Biology, 1985
- The effect of induction of hemoglobin synthesis in cultured friend cells on the number of initiation sites for replication and transcriptionCell Differentiation, 1984
- Effect of novobiocin on DNA synthesis and structure in human lymphoblastoid cellsBiochemical and Biophysical Research Communications, 1981
- Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.Proceedings of the National Academy of Sciences, 1979