Inhibition of DNA replication by ozone in Chinese hamster V79 cells
- 1 January 1986
- journal article
- research article
- Published by Taylor & Francis in Journal of Toxicology and Environmental Health, Part A
- Vol. 17 (1) , 119-128
- https://doi.org/10.1080/15287398609530807
Abstract
DNA replication in Chinese hamster lung fibroblasts, line V79, was depressed in a dose‐dependent manner over an ozone concentration range of 1–10 ppm. When the cells were exposed for 1 h at concentrations up to 6 ppm, the rate of DNA replication, as measured by [ 3 H]thymidine incorporation, declined further during a 3‐h period immediately following exposure. At higher ozone concentrations, at which more than 99.9% of the cells were killed, no further decline in DNA replication was seen beyond that immediately following exposure. Cultures exposed for 1 h to 10 mM ethyl meth‐anesulfonate or to 10J/m 2 of ultraviolet (UV) light showed a similar progressive decline in the rate of DNA replication. The inhibition of DNA replication by ozone resembled that seen after exposure of cells to chemical mutagens or radiation and did not resemble the inhibition produced by metabolic poisons. The results may indicate that ozone or its reaction products interact directly with DNA in a way that inhibits replication.This publication has 2 references indexed in Scilit:
- The HeLa DNA-synthesis inhibition test as a rapid screen for mutagenic carcinogensMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis, 1982
- Mutagenic effects of ozone on human cells exposed in vivo and in vitro based on sister chromatid exchange analysisEnvironmental Research, 1979