Na+‐dependent high affinity uptake of l‐glutamate in primary cultures of human fibroblasts isolated from three different types of tissue
Open Access
- 14 February 1994
- journal article
- research article
- Published by Wiley in FEBS Letters
- Vol. 339 (1-2) , 50-54
- https://doi.org/10.1016/0014-5793(94)80382-x
Abstract
Cultured human fibroblasts isolated from embryonic muscle, skin and peripheral nerve tissues were found to accumulate [3H]L‐glutamate by a Na+‐dependent uptake process strongly inhibited by several glutamate/aspartate analogues including d‐ and l‐aspartate, d‐ and l‐thero‐3‐hydroxyaspartate and l‐trans‐pyrrolidine‐2,4‐dicarboxylate but not d‐glutamate. It was also reduced by elevated concentrations of K+, Rb+ and Cs+. The values of K m 's were 5–20 μM, well within the ‘high affinity’ region. Variations in the capacity (Vmax) of [3H]l‐glutamate uptake did not correlate with the origin (muscle, skin or nerve tissue) of the fibroblasts. The uptake characteristics suggest that it is mediated by a transport system similar to that commonly observed only in brain tissue.Keywords
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