Stimulation of Cyclic AMP and Progesterone Synthesis by LH, PGE2 and Isoproterenol in the Bovine CL in vitro

Abstract
Isoproterenol, a .beta. adrenergic agonist, stimulates the adenyl cyclase-cyclic[c]AMP system in bovine CL [corpus luteum] slices and increases progesterone production. Propranol, a .beta. adrenergic blocker, inhibited stimulation of cAMP and progesterone synthesis by isoproterenol, suggesting that the bovine luteal cell membrane possesses steroidogenically functional .beta. adrenergic receptors. Since propranolol inhibited LH [luteinizing hormone] and PGE2 [prostaglandin] stimulation of progesterone synthesis, had no effect on cAMP accumulation, then propranolol inhibition of progesterone synthesis by LH and PGE2 probably is not related to .beta. blockade.