The use of mass spectrometry to examine the formation and hydrolysis of the phosphorylated form of phosphoglycerate mutase
- 13 February 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 359 (2-3) , 192-194
- https://doi.org/10.1016/0014-5793(95)00044-a
Abstract
Electrospray mass spectrometry has been used to study the formation and hydrolysis of the phosphorylated forms of two phosphoglycerate mutases. The half-life of the enzyme from Saccharomyces cerevisiae was 35 min at 20°C in 10 mM ammonium bicarbonate, pH 8.0. Addition of 1 mM 2-phosphoglycollate reduced this value by at least 100-fold. The phosphorylated form of the enzyme from Schizosaccharomyces pombe was much less stable with a half-life of less than 1 min. The results are discussed in terms of the kinetic properties of the enzymes. Mass spectrometry would appear to be a powerful method to study the formation and breakdown of phosphorylated proteins, processes which are of widespread significance in regulatory mechanismsKeywords
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