A Method of Insulin Bio-Assay and Its Application to Human Plasma Fractions.

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Abstract
Hypophysectomized, alloxan-diabetic mice of CAF-1 Strain (Jackson Laboratories, Bar Harbor) were used as test animals to detect minute amounts of insulin-like activity. Suppression of alimentary hyperglycemia was used as the index of activity. Blood samples from the tail vein was analyzed for glucose; these were drawn 4 [plus or minus] 2 and 65 [plus or minus] 15 minutes after intraperitoneal injection of the test material and simultaneous gastric gavage with 300 mg of dextrin. Four to five milli-units of insulin suppressed completely the marked hyperglycemia otherwise observed. Untreated fresh human plasma showed no activity under these conditions. Plasma fractions, separated by precipitation with Zn and representing 5-fold concentration of the material, were shown to have insulin-like activity, particularly the fraction rich in globulin.

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