Effect of root to solution ratio in ion absorption experiments

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Abstract
Large differences in the absorption behavior of excised roots of barley (Hordeum vulgare) are caused by changing the root to solution ratio. The effect can be ascribed to changes in pH as a result of unequal cation and anion absorption and to the liberation of absorption modifying substances from the root. Ca and probably PO4 are the most important of these. The amount of Ca liberated from the root was shown to be proportional to the root to solution ratio and occurred in amounts sufficiently large to affect absorption. The effect of such influences raises the question of the validity of certain types of kinetic studies which have been applied to the ion absorption process.