Probestin, a new inhibitor of aminopeptidase M, produced by Streptomyces azureus MH663-2F6. I. Taxonomy, production, isolation, physico-chemical properties and biological activities.
- 1 January 1990
- journal article
- research article
- Published by Japan Antibiotics Research Association in The Journal of Antibiotics
- Vol. 43 (2) , 143-148
- https://doi.org/10.7164/antibiotics.43.143
Abstract
Probestin has been isolated as part of a program designed to find microorganism-produced inhibitors of aminopeptidase M from Streptomyces azureus MH663-2F6. It was purified by use of column chromatography of Amberlite XAD-4, silica gel, YMC-gel, Toyopearl HW-40, YMC D-ODS-5 (HPLC) and then isolated as colorless powders. Probestin is competitive with the substrate, and the inhibition constant (Ki) of it was 1.9 .times. 10-8 M.This publication has 3 references indexed in Scilit:
- Probestin, a new inhibitor of aminopeptidase M, produced by Streptomyces azureus MH663-2F6. II. Structure determination of probestin.The Journal of Antibiotics, 1990
- Composite effects of actinonin when inhibiting enkephalin-degrading enzymesEuropean Journal of Pharmacology, 1987
- Identification of aminopeptidase M as an enkephalin-inactivating enzyme in rat cerebral membranesBiochemistry, 1985