FACTORS INFLUENCING THE UPTAKE OF DNA BY NEUROSPORA-CRASSA

  • 1 January 1978
    • journal article
    • research article
    • Vol. 13  (4) , 259-267
Abstract
Under optimal conditions intact N. crassa cells incorporated nearly the same amount of 3H-labeled DNA as that of the endogenous DNA content of the cells. After 18 h of incorporation more than 80% of the radioactivity was retained in the cells. A maximum uptake of exogenous DNA occurred at 28.degree. C, pH 6.35, in the presence of 100 mM Ca when the concentration of donor DNA was 150 .mu.g/ml. Denatured DNA was incorporated at a higher rate than native DNA. The uptake of DNA by N. crassa is probably a biologically active process.