Microvascular remodeling and accelerated hyperemia blood flow restoration in arterially occluded skeletal muscle exposed to ultrasonic microbubble destruction
Open Access
- 1 December 2004
- journal article
- Published by American Physiological Society in American Journal of Physiology-Heart and Circulatory Physiology
- Vol. 287 (6) , H2754-H2761
- https://doi.org/10.1152/ajpheart.00144.2004
Abstract
We showed previously that microbubble destruction with pulsed 1-MHz ultrasound creates a bioeffect that stimulates arteriogenesis and a chronic increase in hyperemia blood flow in normal rat muscle. Here we tested whether ultrasonic microbubble destruction can be used to create a microvascular remodeling response that restores hyperemia blood flow to rat skeletal muscle affected by arterial occlusion. Pulsed ultrasound (1 MHz) was applied to gracilis muscles in which the lateral feed artery was occluded but the medial feed artery was left intact. Control muscles were similarly occluded but did not receive ultrasound, microbubbles, or both. Hyperemia blood flow and number of smooth muscle (SM) α-actin-positive vessels, >30-μm arterioles, and capillaries per fiber were determined 7, 14, and 28 days after treatment. In ultrasound-microbubble-treated muscles, lateral region hyperemia blood flow was increased at all time points and restored to normal at day 28. The number of SM α-actin vessels per fiber was increased over control in this region at days 7 and 14 but decreased by day 28, when larger-diameter arterioles became more prevalent in the medial region. The number of capillaries per fiber was increased over control only at day 7 in the lateral region and only at days 7 and 14 in the medial region, indicating that the angiogenesis response was transient and likely did not contribute significantly to flow restoration at day 28. We conclude that ultrasonic microbubble destruction can be tailored to stimulate an arteriogenesis response that restores hyperemia blood flow to skeletal muscle in a rat model of arterial occlusion.Keywords
This publication has 36 references indexed in Scilit:
- Hemodynamic Stresses and Structural Remodeling of Anastomosing Arteriolar Networks: Design Principles of Collateral ArteriolesMicrocirculation, 2002
- Vascular Gene Transfer of Phosphomimetic Endothelial Nitric Oxide Synthase (S1177D) Using Ultrasound-Enhanced Destruction of Plasmid-Loaded Microbubbles Improves VasoreactivityCirculation, 2002
- Influence of injection site, microvascular pressureand ultrasound variables on microbubble-mediated delivery of microspheres to muscleJournal of the American College of Cardiology, 2002
- Exogenous application of transforming growth factor beta 1 stimulates arteriogenesis in the peripheral circulationThe FASEB Journal, 2002
- Myocardial contrast echocardiography: Basic principlesProgress in Cardiovascular Diseases, 2001
- Intravenous perfluoropropane-exposed sonicated dextrose albumin produces myocardial ultrasound contrast that correlates with coronary blood flowJournal of the American Society of Echocardiography, 1995
- Measurement of blood flow to the adrenal capsule, cortex and medulla in dogs after hemorrhage by fluorescent microspheresJournal of the Autonomic Nervous System, 1990
- The microsphere method for measuring low blood flows: theory and computer simulations applied to findings in the rat cochleaActa Physiologica Scandinavica, 1987
- A gracilis muscle preparation for quantitative microcirculatory studies in the ratMicrovascular Research, 1978
- Hypoxia in skeletal muscle at rest and during the transition to steady workMicrovascular Research, 1977