Macroscopic and Microscopic Behavior of the Eluent in Liquid Chromatography

Abstract
By injecting an isotope pulse into the eluent of liquid chromatography, it has been shown that the eluent flow does not go into the stationary phase, while each of the molecules in the eluent is replaced by an equivalent molecule originally contained in the stationary phase. Meanwhile, an appropriate method for determining the t0 value is given.

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