Application of an arbitrarily‐primed polymerase chain reaction to mycoplasma identification and typing within the Mycoplasma mycoides cluster

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Abstract
An arbitrarily‐primed polymerase chain reaction (AP‐PCR) was developed using a primer pair, Mlip1 and Mlip4, for members of the Mycoplasma mycoides cluster, a group containing important pathogens of small and large ruminants. Parameters that influence the reproducibility of this assay were optimized: magnesium, primer and template concentrations, and pH. AP‐PCR fingerprinting, carried out on a number of strains of each of the six species or subspecies belonging to the mycoides cluster, allowed the typing of strains within each group. The AP‐PCR assay showed that the cluster can be divided into two groups: (i) high and (ii) no genomic polymorphism variation. In addition, specific polymorphic bands for members of species or subspecies included in this cluster were amplified by this AP‐PCR method, thus allowing their identification.

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