DNA methylation in Trypanosoma cruzi

Abstract
DNA isolated from the protozoan Trypanosoma cruzi has been found to contain 5‐methylcytosine. Analysis of T. cruzi DNA by both HpaII and MspI restriction endonucleases suggests that the sequence ‐CCGG‐ is not methylated. Probably T. cruzi DNA also contains N6‐methyladenine. This report constitutes the first clear demonstration of the presence of methylated bases in the nuclear DNA from trypanosomes.