Sephadex LH-20 Cleanup, High Pressure Liquid Chromatographic Assay, and Fluorescence Detection of Zearalenone in Animal Feeds
Open Access
- 1 May 1980
- journal article
- research article
- Published by Oxford University Press (OUP) in Journal of AOAC INTERNATIONAL
- Vol. 63 (3) , 642-646
- https://doi.org/10.1093/jaoac/63.3.642
Abstract
A high pressure liquid chromatographic (HPLC) method is described to determine zearalenone in animal feeds at levels as low as 0.01 ppm. Samples are extracted with chloroform-ethanol and initially purified using a SEP-PAK silica cartridge, followed by column chromatography using Sephadex LH-20. Separation by normal phase HPLC is followed by fluorescence detection. Recoveries at levels of 1.0–0.01 ppm averaged greater than 90%. Confirmation included HPLC analysis of the sample and a zearalenone standard, using 3 different excitation wavelengths, and comparison of fluorescence responses obtained. The method was successfully applied to the analysis of 1 corn and 3 cornmeal samples. Zearalenone was detected in all 4 samples at levels of 0.379–19.2 ppm.This publication has 0 references indexed in Scilit: