PROBE AMPLIFIER SYSTEM BASED ON CHIMERIC CYCLING OLIGONUCLEOTIDES
- 1 August 1990
- journal article
- research article
- Vol. 9 (2) , 142-+
Abstract
Amplification systems are required as part of DNA probe technology, since traditional non-amplified oligonucleotide hybridization using nonradioactive detection methods have detection limits of approximately 108 molecules. We present a probe amplifier technology suitable for use in large-scale automated clinical diagnostic systems. It is fast, sensitive and performs at a constant temperature. The system functions by allowing a single target molecule to act as a catalyst in converting a large number of probe molecules to a unique detectable form. We refer to this catalytic amplification process as the "cycling probe reaction." The basis of the system is an oligomer probe construction consisting of a DNA-RNA-DNA sequence.This publication has 8 references indexed in Scilit:
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