Immunologic Control of the Ascites Form of Murine Adenocarcinoma 755. II. Tumor Immunity Associated With a Friend-Moloney-Rauscher-Type Virus 2

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Abstract
We demonstrated that ADV, a murine C-type virus associated with tumor cells of the ascites form of adenocarcinoma 755 (AD755a), was closely related to the Friend and Rauscher murine leukemia agents, and we explored the role of virus-related antigens in the serum transfer rejection process. The syngeneic mouse anti-AD755a antiserum which was capable of transferring specific tumor immunity could neutralize ADV, Friend murine leukemia virus (F-MuLV), and Rauscher murine leukemia virus (R-MuLV) infectivity, as well as demonstrate group-specific reactivity for the major envelope glycoprotein (gp71) of murine leukemia virus (MuLV) in radioimmunoassay. The neutralizing activity appeared to be mainly type-specific for F-MuLV-R-MuLV-type viruses. The serum could also lyse mouse cells producing F-MuLV, but absorption studies with purified viruses and viral components indicated that this reactivity was only partially directed toward virus structural proteins. Similar absorptions revealed that the immune transfer capacity of the serum could not be removed by antigens present in the intact virus preparations. However, absorption with F-MuLV-infected cells, but not cells expressing AKR MuLV or mammary tumor virus antigens, could eliminate the protective capacity of the anti-AD755a serum. Nevertheless, direct immunization of C57BL/6 mice with F-MuLV could induce immunity to AD755a cells. These results suggested that the AD755a cells expressed at least two types of virus-related antigens: 1) virus structural antigens that were involved in the induction of specific tumor immunity subsequent to direct immunization and 2) virus-related cell-surface antigens that were primarily responsible for the induction of antibodies mediating the protective capacity of the anti-AD755a serum seen upon passive transfer.