Cell aggregates in the soft agar “human tumour stem-cell assay”
Open Access
- 1 December 1982
- journal article
- research article
- Published by Springer Nature in British Journal of Cancer
- Vol. 46 (6) , 880-887
- https://doi.org/10.1038/bjc.1982.297
Abstract
We evaluated colony formation in soft agar by cells obtained after mechanical and/or enzymatic disaggregation of 455 malignant human tumours. Counting and assessment of cell colonies in the agar plates were done by inverted microscopy, computerized image analysis, and inspection of serial photomicrographs of the agar plates. Our results indicate that standard methods of tumour disaggregation did not usually produce single-cell suspensions and that aggregates of tumour cells varying greatly in size were placed in the agar. Most groupings of cells identified as colonies 1-3 weeks after plating arose from enlargement of preexisting aggregates of cells.Keywords
This publication has 5 references indexed in Scilit:
- SENSITIVITY OF HUMAN OVARIAN CARCINOMA CELLS TO INTERFERON AND OTHER ANTITUMOR AGENTS AS ASSESSED BY AN IN VITRO SEMI‐SOLID AGAR TECHNIQUE*Annals of the New York Academy of Sciences, 1980
- Use of an image analysis system to count colonies in stem cell assays of human tumors.1980
- Quantitation of Differential Sensitivity of Human-Tumor Stem Cells to Anticancer DrugsNew England Journal of Medicine, 1978
- Primary Bioassay of Human Tumor Stem CellsScience, 1977
- Agar suspension culture for the selective assay of cells transformed by polyoma virusVirology, 1964