Asp‐193 and Glu‐218 of subunit II are involved in the Mn2+‐binding of Paracoccus denitrificans cytochrome c oxidase
Open Access
- 9 June 1997
- journal article
- Published by Wiley in FEBS Letters
- Vol. 409 (2) , 128-130
- https://doi.org/10.1016/s0014-5793(97)00485-7
Abstract
Cytochrome c oxidase contains a binding site for a non‐redox‐active metal at the interface of subunits I and II, usually a magnesium ion. In Paracoccus denitrificans oxidase, typically 20% may be replaced by manganese, using standard growth media. Site‐directed mutants were constructed in subunit II (D193N and E218Q), and the isolated enzymes analyzed by total‐reflection X‐ray fluorescence spectrometry and EPR. Both mutants show a strong reduction of the manganese stoichiometry and a diminished electron transfer activity, demonstrating that D193 and E218 are involved in the binding of a manganese/magnesium ion in this site.Keywords
This publication has 22 references indexed in Scilit:
- A continuous wave and pulsed EPR characterization of the Mn2+ binding site in Rhodobacter sphaeroides cytochrome c oxidaseBiochemistry, 1995
- Site-directed mutagenesis of cytochrome c oxidase reveals two acidic residues involved in the binding of cytochrome cBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1995
- The terminal oxidases of Paracoccus denitrificansMolecular Microbiology, 1994
- ENERGY TRANSDUCTION BY CYTOCHROME COMPLEXES IN MITOCHONDRIAL AND BACTERIAL RESPIRATION: The Enzymology of Coupling Electron Transfer Reactions to Transmembrane Proton TranslocationAnnual Review of Biochemistry, 1994
- Reduction of CuA induces a conformational change in cytochrome c oxidase from Paracoccus denitrificansBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1992
- Bacillus subtilis expresses two kinds of haem‐A‐containing terminal oxidasesEuropean Journal of Biochemistry, 1991
- STRUCTURE AND FUNCTION OF CYTOCHROME c OXIDASEAnnual Review of Biochemistry, 1990
- Cytochrome c oxidase is three‐copper, two‐heme‐A proteinEuropean Journal of Biochemistry, 1987
- Cytochromecoxidase in prokaryotesFEMS Microbiology Letters, 1987
- Copper and manganese electron spin resonance studies of cytochrome c oxidase from Paracoccus denitrificansBiochimica et Biophysica Acta (BBA) - Bioenergetics, 1981