Effect of storage in media with different ion strengths and osmolalities on human periodontal ligament cells

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Abstract
Abstract – The viability of the periodontal ligament (PDL) cells is critical for a successful healing of replanted exarticulated teeth. It is mainly dependent on the duration of the extra‐alveolar time and the storage medium. Saliva has usually been recommended as the most suitable storage medium, but recent experimental studies indicate that milk is preferable. In the present study the effect on cultured PDL cell of saliva and milk has been compared with some reference media such as tap water or saline by means of a 3H‐uridine leakage test. Storage in milk or saline was found to cause much less 3H‐uridine leakage that storage in saliva or tap water. Cells stored in milk for 60–180min showed about the same leakage as cellsstored in saline or Hanks' balanced salt solucion. Osmolality measurements showed that saliva was hypotonic, while the osmolality of milk ranged with physiological limits. When the osmolality of saliva was increased by addition of NaC1 the leakage of the stored cells decreased to the level of cells stored in 0.9% Nac1 or milk.