Transgenic markers for mammalian chimeras
- 29 February 1988
- journal article
- research article
- Published by Springer Nature in Wilhelm Roux' Archiv für Entwicklungsmechanik der Organismen
- Vol. 197 (2) , 63-65
- https://doi.org/10.1007/bf00375928
Abstract
We describe the construction of aggregation chimeras between normal and transgenic embryos containing multiple copies of mouse β-globin genes. The transgenic component of the chimeras is then detected in tissue sections by a DNA-DNA in situ hybridization technique, using a biotinylated DNA β-globin probe and an avidin-linked alkaline phosphatase detection system. The general advantages of transgenic markers for chimeras are discussed.Keywords
This publication has 9 references indexed in Scilit:
- Localization of low abundance dna sequences in tissue sections by in situ hybridizationJournal of Cell Science, 1986
- IDENTIFICATION OF EMBRYONIC-CELL LINEAGES IN HISTOLOGICAL SECTIONS OF M-MUSCULUS [--] M-CAROLI CHIMERAS1983
- Cell lineage analysis of cerebellar Purkinje cells in mouse chimerasDevelopmental Biology, 1981
- The beneficial effect of EDTA on development of mouse one-cell embryos in chemically defined mediumDevelopmental Biology, 1977
- Labeling deoxyribonucleic acid to high specific activity in vitro by nick translation with DNA polymerase IJournal of Molecular Biology, 1977
- Dominance of AKR Lymphocytes in Tetraparental AKR←⇐CBA-T6T6 ChimaerasNature New Biology, 1973
- Emission Mechanism in PulsarsNature, 1973
- The biochemistry of differentiation of mouse trophoblast: studies on polyploidyDevelopment, 1972