Nucleotide sequence of the asnA gene coding for asparagine synthetase of E.coli K-12
Open Access
- 25 September 1981
- journal article
- research article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 9 (18) , 4669-4676
- https://doi.org/10.1093/nar/9.18.4669
Abstract
We have subcloned the asnA gene of E. coli K-12, a gene coding for asparagine synthetase, from a previously cloned 6 mega-dalton segment of E. coli chromosome containing the DNA replication origin, ori, and asnA. The complete nucleotide sequence of the asnA gene was determined: the region of the structural gene extends 990 base-pairs at nucleotide positions 1434-2423 (see Fig. 3), which codes for a polypeptide of 330 amino-acid residues with a molecular weight of 36,688 daltons. The nucleotide sequences of the promoter and the ribosome-binding site of the gene are also assigned. We discuss the properties of its polypeptide.Keywords
This publication has 21 references indexed in Scilit:
- Codon catalog usage is a genome strategy modulated for gene expressivityNucleic Acids Research, 1981
- The DNA Replication Origin (ori) of Escherichia coli: Structure and Function of the ori-Containing DNA FragmentProgress in Nucleic Acid Research and Molecular Biology, 1981
- Linkage map of Escherichia coli K-12, edition 6.1980
- Nucleotide sequences of trpA of Salmonella typhimurium and Escherichia coli: an evolutionary comparison.Proceedings of the National Academy of Sciences, 1979
- Specific cleavage of coliphage fd DNA by five different restriction endonucleases from Haemophilus genusFEBS Letters, 1973
- Studies on the mechanism of repression of arginine biosynthesis in Escherichia coli: IV. Further studies on the role of arginine transfer RNA repression of the enzymes of arginine biosynthesisJournal of Molecular Biology, 1971
- Purification of Five Leucine Transfer Ribonucleic Acid Species from Escherichia coli and Their Acylation by Heterologous Leucyl-Transfer Ribonucleic Acid SynthetaseJournal of Biological Chemistry, 1971
- The sequence of nucleotides in tRNAIle from E. coli BBiochemical and Biophysical Research Communications, 1971
- The asparagine synthetase of Escherhic coli. I. Biosynthetic role of the enzyme, purification, and characterization of the reaction products.1969
- Studies on polynucleotidesJournal of Molecular Biology, 1967