ANALYSIS OF ANTIBODY-RESPONSE AND IMMUNOGLOBULINS IN SERA OF RABBITS AND GUINEA-PIGS IMMUNIZED WITH COXIELLA-BURNETII

  • 1 January 1977
    • journal article
    • research article
    • Vol. 21  (3) , 246-255
Abstract
Rabbit and guinea pig sera and their immunoglobulin [Ig] fractions (IgM and IgG) were examined by complement-fixation (CF), microagglutination (MA), opsonization-phagocytosis (OP) and serum protection (SP) tests at intervals after immunization with live phase I and phase II C. burnetii suspensions. In general, MA antibodies appeared, but also decreased, earlier than CF antibodies. The anamnestic immune response was higher with lower primary doses. Phase II and phage I CF and MA antibodies as well as phase I opsonins and protective antibodies were found in either Ig fraction depending on post-immunization intervals. At later intervals and especially after the 2nd immunizing dose the levels of protective antibodies in whole sera, but mainly in the IgG fractions, showed a better agreement with those of phase I opsonins rather than of phase I CF and MA antibodies.

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