Immunohistochemical localization and quantification of desmoplakins I & II and keratins 1 and 19 in plastic-embedded sections of human gingiva.
Open Access
- 1 April 1991
- journal article
- abstracts
- Published by SAGE Publications in Journal of Histochemistry & Cytochemistry
- Vol. 39 (4) , 519-528
- https://doi.org/10.1177/39.4.1706376
Abstract
We developed immunohistochemical and image analytical techniques to localize and quantify keratins and desmoplakins in sections of plastic-embedded human gingiva. Acetone fixation followed by plastic embedding of gingiva provided excellent morphology and permitted immunohistochemical detection of keratins 1 and 19 and desmoplakins I & II after 2.5-min trypsin digestion. Quantitative image analysis demonstrated that different volume densities of staining of each marker were associated with specific epithelial strata. Keratin 1 stained most heavily in granular strata, followed by corneal and spinous strata; keratin 19 stained most strongly in the basal layer; desmoplakins I & II stained most strongly in the granular and corneal strata. These findings confirm that variations of keratin and desmoplakin expression in these epithelial are associated with regional patterns of epithelial differentiation.Keywords
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