Isolation of mammalian pol ß type DNA polymerase in shoot tips of germinated seedlings of IR‐8 rice (lOryaza sativa L.)
Open Access
- 1 May 1996
- journal article
- Published by Wiley in IUBMB Life
- Vol. 39 (1) , 117-126
- https://doi.org/10.1080/15216549600201121
Abstract
A single polypeptide of approx. 67 kDa mol.wt. with DNA polymerase activity has been chromatographically purified from shoot tips of 72 hr. grown germinated seedlings of rice (lOryza sativa.L.cv IR‐8). An approx. 4800 fold enrichment of specific activity was measured by the incorporation of 3H‐dTMP into trichloroacetic acid insoluble fraction, using activated calf thymus DNA as template‐primer. The enzyme uses different types of DNA but not RNA as a template. The enzyme requires Mg++, high KCl, and is highly sensitive to dideoxyribonucleoside triphosphate but is unaffected by aphidicolin, suggesting a plant counterpart of mammalian DNA polymerase β. Replication of M13mp18 single stranded DNA by extension of 5′132P‐labeled 17‐mer primer(‐40) showed distributive type of DNA synthesis by the rice DNA polymerase β, which is a characteristic feature of mammalian DNA polymerase ß.Keywords
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