Cryopreservation of 250 strains of Chlorococcales by the method of two-step cooling
Open Access
- 1 March 1978
- journal article
- research article
- Published by Taylor & Francis in British Phycological Journal
- Vol. 13 (1) , 15-24
- https://doi.org/10.1080/00071617800650031
Abstract
A two-step cooling technique is described which allows the successful recovery (>50% viability) of 252 strains of Chlorococcales following freezing to and thawing from -196°C. The method was unsatisfactory with only 32 strains. Survival of cells at -196°C was demonstrated to be independent of the time of storage for a period of two years.This publication has 23 references indexed in Scilit:
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