Evaluation of four assay methods for determination of tobramycin in human serum.
Open Access
- 1 January 1982
- journal article
- research article
- Published by Oxford University Press (OUP) in Clinical Chemistry
- Vol. 28 (1) , 177-180
- https://doi.org/10.1093/clinchem/28.1.177
Abstract
Four assay procedures for tobramycin in serum--enzyme immunoassay (I), substrate-labeled fluorescent immunoassay (II), radioimmunoassay (III), and bioassay (IV)--were compared and evaluated by replicate and analytical recovery studies. I and II were about 50% more precise than III and IV. II was substantially more nearly accurate than the other methods and also gave the best reproducibility (correlation coefficient 0.992 between-day). The least expensive method was IV. Ease of handling favored I and II. Overall, we find II to be the most acceptable procedure for use in the clinical laboratory.This publication has 3 references indexed in Scilit:
- Methods of measuring zones of inhibition with the Bauer-Kirby disk susceptibility testJournal of Clinical Microbiology, 1979
- Assay of serum tobramycin levels with the Bactec 460Antimicrobial Agents and Chemotherapy, 1979
- Correlation of aminoglycoside dosages with serum concentrations during therapy of serious gram-negative bacillary diseaseAntimicrobial Agents and Chemotherapy, 1979