Abstract
The author has developed a new method for determining accurately the phosphatid content of small quantities of tissue. The particular advantage in the method lies in the fact that only a single period of extraction is necessary, thus the method becomes useful in carrying on large series of experiments. Experiments on different types of muscles have shown that the phosphatid content in general parallels the ability to carry on contraction over long periods. Thus the heart muscle of the rabbit gives 0.43% phosphatid-phosphoric acid, the red muscle about 0.173% and the white muscle only 0.128%. It is particularly noteworthy that the total acid-insoluble phosphoric acid in the heart muscle exists as phosphatid phosphoric acid, while in the red muscle, and still more in the white muscle, there is only a slight remainder accounted for as nucleo-protein phosphoric acid. Similar results were obtained with the pig heart. Consequently, the phosphatid content of the heart can be estimated as the difference between the total phosphoric acid and the acid-insoluble phosphoric acid in the tissue.

This publication has 1 reference indexed in Scilit: